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Jun 04 2014

Magnetic Sample PrepTechniques

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Citation: He J, Huang M, Wang D, Zhang Z. Magnetic separation techniques in sample preparation for biological analysis: A review. Journal of Pharmaceutical and Biomedical Analysis.Available online 24 April 2014. In Press, Corrected Proof. http://www.sciencedirect.com/science/article/pii/S0731708514002088

Abstract: Sample preparation is a fundamental and essential step in almost all the analytical procedures, especially for the analysis of complex samples like biological and environmental samples. In past decades, with advantages of superparamagnetic property, good biocompatibility and high binding capacity, functionalized magnetic materials have been widely applied in various processes of sample preparation for biological analysis. In this paper, the recent advancements of magnetic separation techniques based on magnetic materials in the field of sample preparation for biological analysis were reviewed. The strategy of magnetic separation techniques was summarized. The synthesis, stabilization and bio-functionalization of magnetic nanoparticles were reviewed in detail. Characterization of magnetic materials was also summarized. Moreover, the applications of magnetic separation techniques for the enrichment of protein, nucleic acid, cell, bioactive compound and immobilization of enzyme were described. Finally, the existed problems and possible trends of magnetic separation techniques for biological analysis in the future were proposed.

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